本研究主要目的為量測微渠道在不同高度之流場分佈情況，藉由改變微渠道的底層材質（PDMS/Coverglass）觀察二材質因則塔電位（Zeta potential）的差異所造成之流體速度分佈改變，並建立其速度分佈。在本實驗中選擇1× TBE，黏滯度為1 cp為緩衝溶液，並且控制電場強度（5、7.5、10 kV/m）及配合微質點影像測速儀（Micrometer resolution Particle Image Velocimetry, μPIV）量測局部的速度分佈，以探討在不同渠道高度的情形下，DNA分子受水力拉伸的情形。本研究更進一步統計DNA在微渠道中拉伸的長度之分佈，並計算DNA分子的型態變化及應力鬆弛時間，藉此了解不同位置的拉伸情形，進而對DNA分子的在微渠道內的拉伸及形變有更深入的了解。

Abstract
This study aims to measure the flow field distribution in a microchannel with different heights adjusted. Two different materials, PDMS and Coverglass, were used to observe the flow velocity distribution change resulting from the difference in Zeta potential. The velocity distribution data were also obtained. In the experiment, 1× TBE buffer solution with viscosity of 1 cp was used with the electric field intensity controlled under 5, 7.5 and 10 kV/m, respectively. Micrometer resolution Particle Image Velocimetry (μPIV) was used to measure partial velocity distribution in order to explore the hydrodynamic stretch effect on DNA molecules when the microchannel, where the solution was placed, was adjusted to different heights. This study also statistically analyzed the stretch length distribution of DNA molecules in the microchannel and calculated the time of DNA molecule deformation and stress relaxation time in order to understand the stretch condition under different heights as well as the stretch and deformation of DNA molecules in microchannels.

目錄………………………………………………………………i
表目錄……………………………………………………………iii
圖目錄……………………………………………………………iv
符號說明………………………………………………………....vi
中文摘要…………………………………………………………viii
英文摘要…………………………………………………………ix
第一章　序論………………………………………………..……1
1-1 前言………… ……………………………………..……...1
1-2 DNA分子結構與性質………………… ………..…………..1
1-3 機電系統與生醫晶片……………………………..………....2
1-4 背景與目的… …………………………………..…………...4
1-5 文獻回顧…………………………………………………......7
第二章　實驗系統與設備………………………………………18
2-1 微質點影像測速儀系統…………………………….....…...18
2-2 製程設備………………………………………………........19
2-3 直流電源供應器………………………………………........21
2-4 其他實驗週邊設備………………………………………....21
第三章　實驗方法及步驟………...…………………………....30
3-1 微流道設計與製程………………………………....……...30
3-2 工作流體配製………………………………………….......33
3-3 μPIV量測系統建立及原理….………………………..........33
3-4 實驗量測參數………….………………………………......34
第四章　理論分析………….……………………………….....43
4-1 DNA分子流變學拉伸理論…………....…………………..43
4-2 無因次參數分析………………………………………...…44
第五章　誤差分析.......................................................................48
第六章　結果與討論……..…………………………………….52
6-1 μPIV之流場量測…………………….....…………………..52
6-2 DNA分子螢光可視化分析…………………………....…..53
6-3 DNA分子受力分析與拉伸模型分析…………………......55
6-4 速度梯度與剪應力..............................................................57
6-5 DNA分子的平均拉伸比......................................................58
第七章　結論與建議…….……………………………….........84
7-1 結論……………………..………………………………....84
7-2 建議與改進………………………..………………………85
參考文獻……………………………………………………….86
附錄 A…….……………………………………………….……94

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