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博碩士論文 etd-0908103-103743 詳細資訊
Title page for etd-0908103-103743
論文名稱
Title
大鼠腦幹死亡過程差異表現基因之鑑定
Differentially expressed genes in the brain stem of the endotoxemia rat
系所名稱
Department
畢業學年期
Year, semester
語文別
Language
學位類別
Degree
頁數
Number of pages
86
研究生
Author
指導教授
Advisor
召集委員
Convenor
口試委員
Advisory Committee
口試日期
Date of Exam
2003-07-30
繳交日期
Date of Submission
2003-09-08
關鍵字
Keywords
抑制性扣減雜交、內毒素、腦死
brain stem death, LPS, SSH
統計
Statistics
本論文已被瀏覽 5710 次,被下載 13367
The thesis/dissertation has been browsed 5710 times, has been downloaded 13367 times.
中文摘要
近年來的研究發現,經過E.coli lipopolysaccharide (LPS)處理過的Sprague-Dawley大鼠,在整個死亡過程中,測量系統動脈壓(SAP),會觀察到低頻訊號(0-0.8Hz)的變動。低頻訊號的變動分做三期,訊號在一期會降低,二期會增強,第三期又會有降低的趨勢。而低頻訊號認為是來自腦幹,並且和腦幹中的網狀膜腹外側核rostral ventrolateral medulla(RVLM)有密切的關係。為了想要瞭解在低頻訊號變動的過程中,是否具有差異表現的基因存在,並且這些基因是否調控腦幹死亡的過程。我們使用抑制性的扣減雜交suppression subtractive hybridization(SSH),一種找尋差異基因非常有效的方法,並且建立其基因庫,來找出三期個別的差異表現基因。到目前為止,我們發現分別在一期、二期及三期都有差異表現的基因,這些基因都是正向調控的,可能參與大鼠腦幹的死亡過程中的分子調控機制。
Abstract
Abstract
Recent studies demonstrated that LPS treated Sprague-Dawley rats induced a reduction (Phase I), followed by an augmentation (Phase II) and a secondary decrease (Phase III) in the power density of vasomotor components (0-0.8 Hz) in systemic arterial pressure signals. The molecular mechanisms underlie the progression toward death in the brain stem is unclear. In order to find out the differentially expressed genes between LPS-treated RVLM and saline-treated RVLM, we used suppression subtractive hybridization,a method commonly used to search differentially expressed genes, and subtractive cDNA library construction. At present, we have found some differentially expressed genes and these genes are up-regulation expression. These genes may be involved in the progression toward death in the rat brain stem.

目次 Table of Contents
中文摘要1
英文摘要2
緒論3
實驗目的11
材料與方法12
實驗結果26
討論31
參考文獻45
表格與附圖51
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