中文摘要
細胞間白素-1(Interleukin-1, IL-1)家族成員之Interleukin- 1β (IL-1β)胝與細胞間白素受體拮抗子(Interleukin -1 receptor antagonist, IL-1RN)在胃腸黏膜的發炎反應裡扮演著重要的角色。IL-1β具有拮抗胃酸分泌(anti-secretory)和抑制胃部排空的作用，因此，可以作為抗潰瘍及胃部細胞保護劑。IL-1RN是一種自然存在於人體的抗發炎生物素，通過與IL-1受體結合，特異性地阻斷IL-1的活性。幽門螺旋桿菌 (Helicobacter pylori, H. pylori)，是一種革蘭氏陰性細菌，它與胃潰瘍，十二指腸潰瘍，以及胃炎有極為密切的關係。本研究中使用了PCR-RFLP、PCR-VNTR及Real-time PCR等實驗方法，分析123個胃癌病患與225個健康檢查正常人檢體的IL -1β及IL -1RN基因型，配合臨床幽門螺旋桿菌檢查及專業人員一對一的問卷調查，最後以卡方檢定(Statistical Products & Service Solutions, SPSS)、危險指數(odds ratio, OR)及95％ CI (confidence intervals)來做交叉比對統計，以評估個體之IL-1β與IL-1RN基因多型性及幽門螺旋桿菌感染狀況是否與其罹患胃癌有相關性。結果發現，胃癌病人與健檢正常人的IL-1RN基因型分佈有顯著的差異( p = 0.04，X2 = 13.148)，但IL-1β (-31)並沒有顯著的差異( p = 0.298，X2 = 2.418)。攜帶IL-1RN 2R基因型者罹患胃癌之危險率偏高，其危險比值(odds ratio, OR)為4.78 (95% CI : 2.93 ~ 8.75)，且有統計上的差異( p = 0.004)。比較胃癌病人與健檢正常人haplotype的危險比值顯示，基因型為 IL-1RN 2R/ IL-1β (-31) T者罹患胃癌的危險性增高，其危險比值為4.82 (95% CI : 2.38 ~ 7.51)，但沒有統計上的差異( p = 0.253)。除此之外，我們的實驗統計顯示有抽煙習慣又攜帶IL-1β (-31) T基因型的人會增加罹患胃癌的危險性，其危險比值為2.86 (95% CI : 2.06 ~ 4.65)，且有統計上的差異( p = 0.010)。綜合本研究結果得知IL-1RN 2R基因型對罹患胃癌是具有危險性的，再加上受到幽門螺旋桿菌感染及抽煙、喝酒、吃檳榔等習慣對罹患胃癌有加成的效果。由此得知，除了基因型的影響之外，後天的環境及習慣因素也會對罹患胃癌有明顯的危險性。

Abstract
The members of interleukin-1 (IL-1) gene family, interleukin-1β (IL-1b) and Interleukin -1 receptor antagonist (IL-l RN) are cytokines that play a key role in modulating the inflammatory response in the gastrointestinal mucosa. IL-1β

目 錄
中文摘要……………………………………………………… 4
英文摘要……………………………………………………… 6
背景介紹……………………………………………………… 8
實驗目的……………………………………………………… 14
實驗材料……………………………………………………… 15
實驗方法……………………………………………………… 18
結果…………………………………………………………… 22
討論…………………………………………………………… 28
參考文獻……………………………………………………… 31
附表…………………………………………………………… 35
附錄…………………………………………………………….44

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