惡性腫瘤一直高居台灣地區十大死亡原因首位，惡性腦瘤雖在惡性腫瘤中佔較少的比例但治療的效果極不佳。綠茶萃取物中的EGCG可以抑制癌細胞生長，並且促進癌細胞進行細胞凋亡，這個現象已在多個癌細胞模式中被證實，但是在腦瘤細胞還未被透徹的研究。因此，本實驗以人類腦瘤細胞株GBM8401、U87及U251為實驗對象，觀察EGCG對腦瘤細胞產生的影響。經EGCG處理的GBM8401、U87及U251細胞均有明顯被毒殺的現象，其中GBM8401尤其明顯。另外，利用反轉錄聚合酵素連鎖反應以及西方墨點法檢測EGCG處理後GBM8401中多個與細胞凋亡相關的基因及蛋白，結果發現p21、p27及p53均依著EGCG濃度或時間的增加有表現量上升的現象。在固著非依賴性生長分析中也可以看出EGCG可以降低GBM8401細胞形成腫瘤的能力。根據本實驗結果，EGCG對於腦瘤細胞株的生長確實有抑制效果，這說明EGCG可能有治療腦癌或預防癌症方面的潛力。

Cancer has become the major factor causing death in Taiwan. Although brain tumor take a few ratio in cancer, the current therapy shown not effect well. In previous studies, EGCG will promote cancer cells to go apoptosis after treatment with EGCG. But the mechanism in brain tumor is unknown. So, we use EGCG from green tea extract to investigate cell death of human brain tumor cell lines GBM8401, U87MG and U251. The metabolic rates of GBM8401, U87 and U251 are decrease with EGCG treatment, and it is significant in GBM8401. We also examine some genes and proteins related apoptosis by RT-PCR and Western blotting. The accumulation of p21, p27 and p53 are increasing with concentration and time course treatments. EGCG can inhibit the ability of brain tumor to form foci in anchorage-independent growth assay. Our data show that EGCG can inhibit brain tumor cell lines GBM8401, U87 and U251. It is validated that EGCG has the potential of cancer therapy and prevention.

中文摘要................................................1
英文摘要 ...............................................2
壹、 前言 .........................................3
一、 腦瘤...........................................3
二、 細胞週期.......................................4
三、 細胞凋亡.......................................8
四、 兒茶素.........................................11
貳、 材料與法.......................................14
參、 實驗結果與論...................................22
肆、 結論...........................................31
參考文獻..............................................32
表....................................................37
圖....................................................38
附錄..................................................55

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