Transmembrane and coiled-coil domains 1(TMCO1)是一種穿膜蛋白，目
前並沒有任何文獻可以指出TMCO1 的功能為何。而本研究室根據先前生物
資訊學及史丹福微陣列資料庫 (Stanford Microarray Database, SMD) 的探
勘 (data-mining) 與初步的數據分析發現TMCO1，並利用IHC
(immunohistochemistry) 發現TMCO1 在非侵入性的膀胱癌具有高度的表現，
因而想要更進一步的研究TMCO1在膀胱癌中所扮演的角色。 在本研究中，
我們檢視了三株人類膀胱癌細胞分別為、RT4、TSGH8301 和J82 的TMCO1
內生性表示量。 我們發現TMCO1 在RT4 會高度表現而在TSGH8301 表現
量較低。因此接下來我們將抑制RT4 中TMCO1 的表現量觀察TMCO1 對
細胞的影響。在研究中我們發現當TMCO1 表現量下降時會促進細胞快速生
長。

The product of transmembrane and coiled-coil domains 1 (TMCO1) gene is a
member of several eukaryotic proteins with unknown function. In a preliminary
Bioinformatics and Stanford Microarray Database data-mining and some of our
preliminary data, transmembrane and coiled-coil domains 1 (TMCO1)
immunohistochemistry (IHC) was identified to be up-regulated in non-invasive
bladder cancer. To further studies on the functions and regulatory mechanisms
the role of TMCO1 gene. In the present study, we examined the TMCO1
expression levels in human bladder cell lines, RT4, TSGH8301, J82. We
observed TMCO1 high expression in RT4 and low expression in TSGH8301.
We investigate overexpression and knock down of TMCO1in RT4 and
TSGH8301. Further we study the expression change of TOMCO1 how to change
cell function and morphology. In this study, we can get transfection of shRNA
interference targeting TMCO1 in RT4 cell enhanced cell proliferation.

目錄
English abstract---------------------------------------------------------------------- I
中文摘要------------------------------------------------------------------------------- II
英文縮寫表---------------------------------------------------------------------------- III
目錄------------------------------------------------------------------------------------- V
一、 緒論----------------------------------------------------------------------------- 1
二、 材料方法----------------------------------------------------------------------- 6
三、 結果----------------------------------------------------------------------------- 24
四、 討論----------------------------------------------------------------------------- 40
五、 Supplementary Data-------------------------------------------------------- 42
六、 參考文獻----------------------------------------------------------------------- 44

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