hsDC2基因（NM_021227）位在染色體的4q25位置上，此基因的蛋白質產物含有149個胺基酸，大約為16.8 kDa。
經由生物資訊網站的分析，挑選了hsDC2基因上三段非穿膜的區域，來製作hsDC2蛋白的多株抗體。這三段非穿膜的區域分別以大腸桿菌BL21(DE3)來表現GST-hsDC2 a1~31與GST-hsDC2 a54~82以及將合成的hsDC2 a140~149 polypeptide接合到mcKLH上的方式來製備免疫兔子的抗原。兔子經免疫刺激後的血清，先以抗原進行西方墨點法比較免疫前後血清對抗原的專一性。接著利用不同的萃取液萃取細胞的全蛋白，進行西方墨點法確定經由不同的蛋白萃取液所得到的訊號是一致的。此外，將DC2基因接上帶有標誌的載體，利用電穿孔的方法，送入真核細胞過量表現DC2蛋白，利用純化後的抗體進行西方墨點法，來偵測DC2蛋白過量表現的訊號。並且在電穿孔的實驗中意外發現，細胞經電擊後會受到刺激誘導DC2的表現。
最後，將所製作的抗體用於檢測受藥物刺激分化的U937血癌細胞株，去瞭解在細胞分化過程中hsDC2蛋白質的表現情形。

The hsDC2, an unknown gene, was located on chromosome 4q25. The genetic protein product contained 149 amino acids with the molecular weight of 16.8 kDa.
By bioinformatics, three predicted non-membrane portion of hsDC2 gene were designed to immune rabbit. The hsDC2 a1~31and hsDC2 a54~82 gene were inserted in pGEX6p-1 that expressed as fusion protein with GST. The other, hsDC2 a140~149, was synthesized with additional cycteine for conjugation with mcKLH at N-terminal. After four times subcutaneous injection, the antiserum was blooded from rabbits. The antibody was purified from the antiserum by protein A beads and confirmed with antigen by western blotting. The identified antibody was used to examine the expression of hsDC2 protein during U937 differentiation induced by RA and PMA.

目錄
中文摘要-------------------------------------------------------------------------------1
英文摘要-------------------------------------------------------------------------------2
壹、緒論-------------------------------------------------------------------------------3
貳、實驗目的-------------------------------------------------------------------------9
參、實驗材料與方法----------------------------------------------------------------10
肆、結果與討論---------------------------------------------------------------------23
伍、結論-------------------------------------------------------------------------------30
陸、參考資料------------------------------------------------------------------------31
柒、附表-------------------------------------------------------------------------------35
捌、附圖------------------------------------------------------------------------------36

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