核磁共振光譜和微影像技術，由於其非侵入性和靈活性，可對活體做即時的光譜和影像記錄而不會破壞其生理結構，已廣泛應用在植物生理學的研究上，包括形態學到水和養份輸送以及代謝的過程等。例如：利用質子密度影像觀察植物形態；T1，T2 加權影像得知植物體內，水在不同空間上的運動，進而得知代謝資訊；擴散加權影像用以了解水及養份的傳輸。
本實驗結合核磁共振光譜和微影像技術， 特別是利用在魔角旋轉條件下得到高解析光譜並結合各種加權影像（橫向/縱向鬆弛，化學位移等），來觀測芝麻發芽各個階段的代謝過程及生理結構變化。通過選擇不同的轉速及溫度進行實驗，希望藉此方法能在觀察到光譜上有化學位移變化或峰群的生成及消失時，搭配影像以及植物生理學的知識，能夠指認出發芽時特定代謝物的空間分佈和隨時間的演化，進而分析其代謝路徑。

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目錄
第一章 緒論………………………………………………………..1
第二章 種子發芽生理學…………………………………………..3
2.1發芽與生長條件……………………………………………………...3
2.2本次實驗的其它因子………………………………………………...5
第三章 NMR/I在植物學上的應用……………………………….6
3.1 植物學上的應用…………………………………………………….6
3.2 植物代謝體學上之應用…………………………………………….6
第四章 實驗材料與方法…………………………….………….…8
4.1 芝麻的營養成分…………………………………………………….8
4.2 儀器與實驗設計…………………………………………………...11
4.2 實驗所使用的脈衝序列與參數設定…………………………...…14
4.2.1 脈衝序列……………………………………………………14
4.2.2 參數設定……………………………………………………16
4.3 MAS(Magic-angle spinning)魔角旋轉……………….…………….17
第五章 結果與討論………………………………………………19
5.1 芝麻籽在水溶液培養下固態魔角旋轉光譜之結果分析……...…19
5.1.1 芝麻籽在純水培養下之液態光譜結果分析………………31
5.1.2 芝麻籽在純水培養下之固態魔角旋轉T1結果分析………39
5.1.3 芝麻籽在水溶液培養下之固態魔角旋轉T2結果分析………42
5.2 芝麻籽在IAA水溶液培養下之固態魔角旋轉光譜結果分析…..…..45
5.2.1 芝麻籽在IAA水溶液培養下之液態光譜結果分析……….…50
5.2.2 芝麻籽在IAA水溶液培養下之固態魔角旋轉T1結果分析…..57
5.2.3 芝麻籽在IAA水溶液培養下之固態魔角旋轉T2結果分析.....60
5.3 芝麻籽於不同發芽時期之質子密度影像……………………….…..63
5.4 芝麻於不同發芽時期之 R2 Map…………………………………....69
5.5 芝麻於不同發芽時期之 R1 Map………………………………….…78
第六章 結論與未來展望……………………………………………87
6.1 結論………………………………………………………….......……87
6.2未來展望………………………………………………………………88
參考文獻…………………………………………………………………..89

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