摘要 -1

小鼠感染廣東住血線蟲後，主要引起體液性免疫反應。本研究針
對初次與二次感染所引起之全身與局部體液性免疫反應作比較性的
探討。C57BL/6J小鼠感染20隻第三期幼蟲八週後，以相同劑量進
行二次感染。在初次感染後，血清IgM、IgG和IgE在感染後第二週
即出現，IgG1與IgG2b則在第四週起升高，上述各型抗體效價隨感
染時程而持續增高；IgG2a則無升起。在腦組織浸泡液中，IgM、IgG1
在第四週高起，IgG在第六週上升，IgG2b僅在第六週有高起，其他
階段並無有意義的升起，IgG2a與IgE則無顯著變化。二次感染後，
血清IgM有顯著升高，腦組織浸泡液中之IgG1卻降低。以西方墨點
法分析，發現腦組織浸泡液之IgG1、IgE失去對第五期蟲體體抗原
及排泌抗原中一42 kDa分子的辨識能力。這些差異或許可運用於
小鼠二次感染廣東住血線住蟲後的早期診斷。

摘要 -2

以放射造影技術拍攝感染廣東住血線蟲之小鼠顱骨側面影像，測量
其顱骨定點之長高取比值得頂額指數（parietofrontal index, PI）。
與正常小鼠相比較，結果發現小鼠在感染廣東住血線蟲60天後顱頂
均有明顯突起的現象，而此現象於感染後二到四週間開始發生。此
結果或許可以應用作為小鼠感染廣東住血線蟲後，其顱骨隨感染時
程改變的外在診斷參考。

Abstract -1

The immune response occurred in the mice infected with
Angiostrongylus cantonensis was mainly humoral immune
response. This study was designed to compare the systemic and
localized humoral immune responses occurred after primary and
secondary infections in C57BL/6J mice. Eight weeks after the
primary infection with 20 third-stage larvae, each mouse received a
second inoculation of the same dosage. Specific serum IgM, IgG
and IgE were found in the second week after primary infection.
However, the titers of IgG1 and IgG2b increased at the fourth week
after primary infection. Antibodies of these mentioned increased
continuously as the progress of infection. On the other hand, the
IgM and IgG1 titers increased in brain tissue infusion since the forth
week after primary infection, while the titer of IgG start to elevate at
the sixth week. Nevertheless, the increase of IgG2B was only
noticed at the sixth week and no significant change was observed
for IgG2a and IgE. After the secondary infection, serum IgM titers
increased while the titer of IgG1 in the brain tissue infusion
decreased. Results of Western blot showed that IgG1and IgE in the
brain tissue infusion lost the ability to recognize a 42 kDa molecule
of the somatic and excreting-secreting antigens of fifth-stage
larvae. These variations could be used in the diagnosis of the early
stage of mice that re-infected with Angiostrongylus cantonensis. Abstract -2

The radiographic lateral views of the skulls of the mice infected
with Angiostrongylus cantonensis were taken. Thus, the
parietofrontal index ( PI ) was obtained by measuring and
calculating the distances among specific positions on their skulls.
Compared with the controls, a significant elevation over the top of
the crania of the cases was observed sixty days post-infection. In
addition, the phenomenon emerged apparently during the second
to the fourth week post-infection. These findings are able to be
applied as the external diagnostic references for the infection
course of mice infected with Angiostrongylus cantonensis.

目次 -1

•摘要......................................................2
•Abstract................................................3
•目次......................................................4
•圖表次..................................................5
•前言......................................................7
•材料與方法..........................................13
•結果......................................................20
•討論......................................................28
•參考文獻..............................................62
•圖表......................................................32

目次 -2

•摘要......................................................50
•Abstract................................................51
•目次......................................................52
•圖表次..................................................53
•前言......................................................54
•材料與方法..........................................56
•結果......................................................58
•討論......................................................60
•參考文獻..............................................62
•圖表......................................................67

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