台灣西海岸具有廣大淺灘，適於淺海養殖，北由新竹香山南至台南及澎湖均有養殖牡蠣，近年來本省養殖牡蠣有殼空腔化（Chambering）的現象。所謂空腔化就是牡蠣殼在形成時殼層與殼層間有空隙產生之狀況稱之；本省養殖牡蠣早已被發現有此現象，但缺乏相關研究。因此，本論文即針對牡蠣殼空腔化及衍生之相關課題進行研究。實驗分三部分進行：1. 評估空腔化指標計算方法及其與牡蠣成長之關係; 2. 空腔化對牡蠣被掠食之影響; 3. 空腔化與重金屬污染相關性探討。分別以STI值（Shell thickness index）、密度測量法、擊碎測量法及影像數位測量法計算空腔化之情形；比較不同空腔化計算方式及其和牡蠣成長CI值（condition index）的關係。結果顯示STI值及密度是牡蠣殼空腔化較佳之指標;當牡蠣STI值愈小或是密度愈小，表示空腔化愈大。在牡蠣被蚵岩螺掠食之影響實驗中，則顯示牡蠣殼密度愈小，被蚵岩螺攝食鑽孔的深度愈深的趨勢；而重金屬總濃度(包括Cu、Zn、Mn、Cd、Fe)和空腔化未見有統計上之相關性。

Oyster (Crassostrea gigas) culture has been operating on the west coast of Taiwan for two hundred years. In recent years, the occurrence of shell chambering in oysters was noticed, but lack of scientific studies. This study concentrated on: 1. Evaluation of shell chambering methods. 2. Possible relationship between chambering and Thais clavigera predation. 3. Relationships between chambering and heavy metals. The results suggested that shell thickness index (STI) and density reflect chambering status adequately. There was a negative trend between depth of drilling hole by T. clavigera and density of oyster shell, but not significantly. Amount of total heavy metals did not appear significantly related to shell chambering neither.

目錄
章次 頁次
中文摘要……………………………………………………………………………Ⅰ
英文摘要…………………………………………………………………………….II
目錄…………………………………………………………………………………Ⅲ
表目錄………………………………………………………………………………Ⅳ
圖目錄………………………………………………………………………………V
一、前言……………………………………………………………………………1
二、材料與方法……………………………………………………………………4
三、結果……………………………………………………………………………11
四、討論……………………………………………………………………………18
參考文獻……………………………………………………………………………21
附錄…………………………………………………………………………………62

中華民國台灣地區漁業年報1985∼2004。台灣省農林廳漁業局。
行政院環境保護署環境檢驗所。魚介類中鎘、鉻、銅、鎳、鉛及鋅含量方法—電熱式原子吸收光譜法。NIEA CA304.00B，2002。
吳靖穎。2004。台灣西海岸蚵岩螺族群Ark (arginine kinase)同功酵素之時空變化。國立中山大學海洋生物研究所碩士論文。46頁。
胡舜智。1976。 淺海完全養殖。 徐氏基金會出版。
洪英女。2003。雲林海域底拖漁獲物體內中金屬含量之研究。國立中山大學海洋資源研究所碩士論文。115頁。
陳孟仙。2001。 牡蠣重金屬蓄積與食用安全性。從環境品質談牡蠣產業發展的前景座談會，13-26。
黃建華2003。簡易魚苗自動點算系統設計。國立中山大學海洋生物研究所碩士論文。52頁。
楊夢南，巫文隆。1996。 台灣長牡蠣(Crassostrea gigas Thunberg)基礎生物學的研究。 國立台灣大學漁業科學研究所碩士論文。134頁。
劉莉蓮。2001。 牡蠣養殖的現況與問題。 從環境品質談牡蠣產業發展的前景座談會。1-9。
劉正義，朱玉，張聰洲。1990。 台灣養殖牡蠣之形態學及形態病理學。 水產動物防疫手冊。1-16。
Almeida, M.J., J. Machado and J.Coimbra 1999 Growth and Biochemical composition of Crassostrea gigas (Thunberg) and Ostrea edulis (Linnê) in two estuaries from the north of Portual. Journal of Shellfish Research, 18:139-146.
Belcher, A.M., X.H.Wu, R.L. Christensen, P.K. Hansma, G.D. Stucky and D.E. Morse 1996 Control of crystal phase switching and orientation by soluble mollusk-shell proteins. Nature 381:56-58.
Brancato, M.S., R.D. Cardwell and S. Mckay 2000 Chambering in Pacific oyster, Crassostrea gigas , and its relationship to tributyltin. Marine Environmental Research, 50:427.
Dyrynda, E.A. 1992 Incidence of abnormal shell thickening in the pacific oyster Crassostrea gigas in Poole harbour (UK), subsequent to the 1987 TBT restrictions. Marine Pollution Bulletin, 4:156-163.
Han, B.C. and T.C. Hung 1990 Green oyster caused by copper pollution on the Taiwan coast. Environmental Pollution, 65:347-362.
Han, B.C., W.L. Jeng, T.C. Hung, Y.C. Ling, M.J. Shieh and L.C. Chien 2000 Estimation of mental and organochlorine pesticide exposures and potential health threat by consumption of oysters in Taiwan. Environmental Pollution, 109:147-156.
His, E. and R. Robert 1987 Comparative effect of two antifouling paints on the oyster Crassostrea gigas. Marine Biology, 95:83-86.
Hsia, M.P. and S.M. Liu 2003 Accumulation of organotin compounds in Pacific oysters, Crassostrea gigas, collected from aquaculture sites in Taiwan. The Science of the Total Environment, 313:41-48.
Lin, S. and I-JY Hsieh 1999 Occurrences of green oyster and heavy metals contaminant levels in the Sien-San area, Taiwan. Marine Pollution Bulletin, 38:960-965.
Marcus, J. M., G. I. Scott and D.D. Heizer 1989 The use of oyster shell thickness and condition index measurements as physiological indicators of no heavy metal pollution around three coastal marinas, Journal of Shellfish Research, 8:87-94.
Okoshi, K., K. Mori and T. Nomura 1987 Characteristics of shell chamber formation between the local races in the Japanese ouster, Crassostrea gigas. Aquaculture, 67:313-320.
Okoshi, K., A. Iida and M. Ishikawa 1995 Coexistence of two CaCO3 polymorphs in growth front of paper shell obtained from chambered oyster, Crassostrea gigas. Journal of Marine Biotechnology, 2:89-93.
Okoshi, K. and W. Sato-Okoshi 1996 Biomineralization in molluscan aquaculture growth and disease. Bulletin de l'Institut Oceanographique, Monaco, n special, 14,4.
Rainer, J.S. and R. Mann 1992 A comparison of methods for calculating condition index in eastern oyster, Crassostrea virginica (Gmelin,1791). Journal of Shellfish Research, 11:55-58.
Roper, D.S., R.D. Pridmore, V.J. Cummings and J.E. Hewitt 1991 Pollution related differences in condition cycles of Pacific oysters Crassostrea gigas from Manukau harbour, New Zealand. Marine Environmental Research, 31:197-214.
Soong, K., L.L. Liu and J.L. Chen 1992 Regional differences in oyster (Crassostrea gigas Thunberg) size and shape in Taiwan. Bulletin of the Institute of Zoology Academia Sinica, 31:111-119.
Stephenson, M. 1991 A field bioassay approach to determining tributylin toxicity to oysters in California. Marine Environmental Research, 32:51-59.
Thomopson, J.B., G.T. Paloczi, J.H. Kindt, M. Michefelder, B.L. Smith, G. Stucky, D.E. Morse and O.K. Hansma 2000 Direct observation of the transition from Calcite to Aragonite growth as induced by abalone shell proteins. Biophysical Journal, 79:3307-3312.
Waldock, M.J. and J.E. Thain 1993 Shell Thickening in Crassostrea gigas : organotin antifouling or sediment induced? Marine Pollution Bulletin, 14:411-415.