鯽魚膽 (Pluchea indica (L.) Less.) 為菊科灌木植物，廣泛生長在台灣西部沿海。先前的研究指出鯽魚膽具有抗發炎、抗潰瘍及抗微生物等活性。此研究乃探討鯽魚膽根與葉之水萃物對癌細胞株的影響。在GBM8401、HeLa、J5三株癌細胞株中，GBM8401、HeLa細胞數明顯比對照組低，表示鯽魚膽可能抑制癌細胞生長。藉由MTT assay、MTS assay、細胞群落形成分析及細胞移行分析等實驗結果，可看出鯽魚膽根與葉之水萃物對腦癌細胞株GBM8401及子宮頸癌細胞株HeLa 具有抗增生之活性。

Pluchea indica (L.) Less. is a shrub of the family Compositae and is widespread along the western coast in Taiwan. Previous studies indicated that the components of Pluchea indica have potent anti-inflammatory, anti-ulcer and antimicrobial activities. In the present study, the effects of aqueous extract of roots and leaves from P. indica on the cancer cell lines were investigated. Various experimental approaches including cell growth curves, MTT assay, MTS assay, focus formation assay and cell migration assay were performed on the aqueous extract-treated cancer cells. Our results demonstrate the aqueous extract of P. indica induced anti-proliferation activity on GBM8401 and HeLa cancer cell lines.

中文摘要……………………………………………………………… 1
英文摘要.............................................………………………………... 2
前言.................................................…………………………………... 3
研究目的.............................................………………………………... 8
材料與方法.............................................…………………………….. 9
細胞培養……………………………………………………. 9
鯽魚膽水萃物製備方式………………………………….. 10 生長曲線…………………………………………………….10
細胞存活率分析……………………………………………11
細胞移行分析………………………………………………13
評估鯽魚膽根與葉水萃物與抗癌藥物 5-fluorouracil 對癌細胞的協同效應……………………………………..14
細胞群落形成分析………………………………………..14
結果與討論……………………………………………………16

鯽魚膽根與葉水萃物處理不同癌細胞株後細胞的生長情形……………………………………………………………16
以MTT assay的方法來評估癌細胞株在水萃物處理後的代謝活性…………………………………………………..17
細胞群落形成分析…………………………………………18
移除鯽魚膽根水萃物影響後GBM8401 生長回復狀況之探討………………………………………………………….19
細胞移行分析………………………………………………20
以MTS assay的方法來評估正常人類纖維母細胞株及癌細胞株在水萃物處理後的代謝活性………….22
評估鯽魚膽根與葉水萃物與抗癌藥物5-fluorouracil對癌細胞的協同效應…………………………………………….23
結論…………………………………………………………..25
參考文獻…………………………………………………….26
附圖……………………………………………………………….30

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