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博碩士論文 etd-0915106-123143 詳細資訊
Title page for etd-0915106-123143
論文名稱
Title
病原感染對石斑魚血液及血清之影響
Effects of pathogen on blood and serum chemistry of grouper
系所名稱
Department
畢業學年期
Year, semester
語文別
Language
學位類別
Degree
頁數
Number of pages
66
研究生
Author
指導教授
Advisor
召集委員
Convenor
口試委員
Advisory Committee
口試日期
Date of Exam
2006-07-31
繳交日期
Date of Submission
2006-09-15
關鍵字
Keywords
血液、石斑魚、血清
Photobacterium damselae subsp. damselae, serum, blood, grouper
統計
Statistics
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中文摘要
本研究目的在於建立石斑魚血液、血清的相關資料,期望這些資料能有助於及早發現石斑魚的病情,達到及早治療的效果,以減少病害造成的損失。
實驗共分成三大部分:第一部分進行各種體重大小的健康點帶石斑(Epinephelus coioides)血液及血清資料之建立;第二部份則針對罹患不同病原的點帶石斑石斑魚(E. coioides),包括罹患指環蟲(Dactylogurus)、車輪蟲(Trichodinla)、神經壞死病毒(Nervous necrosis virus, NNV)以及神經壞死病毒與卵圓鞭毛蟲(Amyloodinium ocellatum)混合感染的點帶石斑石斑魚進行抽血檢測;第三部分則是以Photobacterium damselae subsp. damselae感染棕點石斑(E. fuscoguttatus)後,進行血液及血清的檢測。
在不同體重大小的健康點帶石斑石斑魚方面,經採血分析後發現,點帶石斑石斑魚白血球之數目會清楚的分為70-90 ´ 103 cell/ml與110-140 ´ 103 cell/ml二大範圍;而紅血球數範圍則介於1.85-3.21 ´ 106 cell/ml間。血清酵素麩氨酸-草醋酸轉氨基酶(Glutamic oxalacetic transaminase, GOT)含量在0-45 IU/L間;麩氨酸-焦葡萄酸轉氨酶(Glutamic pyruvic transaminase, GPT)含量則在0-350 IU/L間,個體之間的差異很大。總蛋白質與白蛋白量分別在3.8- 5.6與0.7-1.6 g/dL間,範圍變化較小。
在罹患不同病原部分,以遭受神經壞死病毒與指環蟲感染之點帶石斑石斑魚的血液變化較明顯,白血球的數量均明顯增加(P<0.05),顆粒球(Granulocyte)則明顯下降(P<0.05)。離子分析方面,受神經壞死病毒感染之點帶石斑石斑魚,其K+偏高(P<0.05),血液的pH下降,尤以感染嚴重瀕臨死亡之點帶石斑石斑魚越明顯。而受指環蟲感染之點帶石斑石斑魚其K+偏高,Ca2+則明顯低於(P<0.05)健康魚。遭車輪蟲感染之點帶石斑石斑魚其Ca2+明顯高於(P<0.05)健康魚,pH值則下降。
血球形態方面,以發光弧菌(Photobacterium damselae subsp. damselae)感染棕點石斑石斑魚(E. fuscoguttatus)後,血球中之單核球(Monocyte)大小,在感染後第8至16小時均明顯大於控制組(P<0.05);而感染組之嗜中性球(Neutrophil)大小則在第4小時明顯大於控制組(P<0.05);嗜酸性球(Eosinophil)大小則是在第16小時大於控制組(P<0.05)。
Abstract
The purpose of this study was for building the data on blood and serum chemistry of grouper ‚ and expect these data can be helpful to know the fish condition in early stages‚ in order to remedy as soon as possible and decrease the loss that caused of fish disease.
Three experiments were conducted to establish the data on blood and serum chemistry of grouper. In the first experiment‚ the clinical chemistry including red and white blood cell counts‚ Glutamic oxalacetic transaminase (GOT)‚ Glutamic pyruvic transaminase (GPT)‚ total protein‚ albumin and blood ion of Na‚ K and Ca were built in different size of healthy orange spotted grouper(Epinephelus coioides). The results showed that the white blood cell counts was divided into two major parts 70-90´103 cell/ml and 110-140´103 cell/ml. Red blood cell counts was landed on 1.85-3.21´106 cell/ml. The range of GOT and GPT were 0-45 IU/L and 0-350 IU/L. Total protein and albumin were 3.8-5.6 and 0.7-1.6 g/dL.
In the second experiment&#8218; the clinical chemistry of orange spotted grouper after infected by dactylogurus&#8218; trichodinla&#8218; nervous necrosis virus (NNV) and NNV with Amyloodinium ocellatum were collected and compared with normal orange spotted grouper. The white blood cell counts and the concentration of K+ in the infected NNV and dactylogurus of fish were significantly higher (P<0.05) than normal fish&#8218; while the granulocyte in infected fish was significantly lower (P<0.05) than normal fish. In the dactylogurus and trichodinla infections of fish&#8218; the concentration of Ca2+ was significantly higher (P<0.05) than normal fish.
In the third experiment&#8218; the size of monocytes and neutrophils of brown marbled grouper(E. fuscoguttatus) were grown in 8 hours and 16 hours&#8218; respectively&#8218; after challenged with Photobacterium damselae subsp. damselae&#8218; and the eosinophils size of challenge group fish bigger (P<0.05) than control group fish after challenged for 16 hours.
目次 Table of Contents
謝辭……………………………………………………………………………………… Ⅰ
中文摘要………………………………………………………………………………… Ⅱ
英文摘要………………………………………………………………………………… Ⅳ
目錄……………………………………………………………………………………… Ⅴ
表目錄…………………………………………………………………………………… Ⅵ
圖目錄…………………………………………………………………………………… Ⅷ
前言……………………………………………………………………………………… 1
文獻整理………………………………………………………………………………… 3
材料與方法……………………………………………………………………………… 8
結果……………………………………………………………………………………… 13
討論……………………………………………………………………………………… 15
參考文獻………………………………………………………………………………… 17
表………………………………………………………………………………………… 24
圖………………………………………………………………………………………… 47
附錄……………………………………………………………………………………… 56
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