Klebsiella oxytoca SYSU-011是一株由電鍍工廠廢水中分離出之氰化物分解菌。在本研究中，我們發現K. oxytoca可以利用四氰化鎳{K2[Ni(CN)4]}(TCN)作為其生長時唯一的氮源，而且無論在氧氣充足（D.O.＝100％）或氧氣缺乏（D.O.＝0％）的環境下均可以分解TCN。若添加較高濃度的銨離子(5 mM)則會抑制TCN的分解。當細菌分解TCN時發現溶液中有綠色沈澱物的產生，經遠紅外線光譜分析後得知其為TCN的代謝產物-二氰化鎳。在靜止細胞實驗中發現銨離子隨著TCN的降解而產生。此外，若在靜止細胞實驗中加入葡萄糖則可以大為促進TCN之分解速率。本實驗證明nitorgenase是K. oxytoca中具有氰分解活性的酵素。而銨離子能對nitrogenase造成抑制作用，TCN及KCN則可誘導出nitrogenase的活性。

The cyanide-degrading bacterium Klebsiella oxytoca SYSU-011 was isolated from the waste water of a metal-plating plant. In this study, we found out that K. oxytoca was capable of utilizing tetracyanonickelate {K2[Ni(CN)4]}(TCN) as its sole nitrogen source. This organism could degrade TCN both aerobically (D.O.＝100％) and anaerobically (D.O.＝0％).The addition of ammonia (5 mM) in the growth medium would inhibit TCN-degrading. The TCN-degrading by-product, a greenish precipitate, was found in the spent medium and was identified as nickel cyanide [Ni(CN)2] by FT-IR spectroscopic studies. Ammonia was demonstrated as a product of the TCN-degrading process by K. oxytoca resting cells. The addition of glucose could greatly enhance the TCN-degradation. Nitrogenase was found to be the cyanide degrading enzyme in this organism. The activity of nitrogenase was inhibited by ammonia but could be induced by the addition of TCN or KCN.

中文摘要………………………………………..……………...I
英文摘要……………………………………….….…………..II
壹. 前言…………..…………………………….……………...1
貳. 研究目的.……..……...……………………….….………12
參. 材料與方法...………………………………………….…13
肆. 結果與討論...………………………………………….…22
伍. 結論………………………………………………………33
陸. 圖表…………………………………………….…….…..35
柒. 參考文獻……………………..………………….…….…54

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