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博碩士論文 etd-1103113-002634 詳細資訊
Title page for etd-1103113-002634
論文名稱
Title
以三種軟體動物生物指標應用於台灣西南海域汙染之可行性探討
Evaluation the use of three mollusk biomarkers to marine pollution in southwestern Taiwan
系所名稱
Department
畢業學年期
Year, semester
語文別
Language
學位類別
Degree
頁數
Number of pages
62
研究生
Author
指導教授
Advisor
召集委員
Convenor
口試委員
Advisory Committee
口試日期
Date of Exam
2013-10-17
繳交日期
Date of Submission
2013-12-03
關鍵字
Keywords
環境生物監測、葡萄牙牡蠣、綠殼菜蛤、生物指標、蚵岩螺
Thais clavigera, Crassostrea angulata, Perna viridis, environmental monitoring, biomarker
統計
Statistics
本論文已被瀏覽 5835 次,被下載 201
The thesis/dissertation has been browsed 5835 times, has been downloaded 201 times.
中文摘要
本研究探討三種生物指標:溶酶體膜不穩定度、肥滿度指數與一維電泳蛋白質體表現應用於西南海岸環境監測之可行性。實驗分三部分進行,第一部份為比較不同時間地點(高雄港、大鵬灣與西子灣)綠殼菜蛤溶酶體膜不穩定度之差異,結果顯示時空變化皆影響綠殼菜蛤溶酶體膜不穩定度。第二部分為比較不同地點葡萄牙牡蠣與蚵岩螺溶酶體膜不穩定度與肥滿度指數之差異,結果顯示葡萄牙牡蠣與蚵岩螺之溶酶體膜不穩定度有地點間之差異;肥滿度指數則是葡萄牙牡蠣地點間有顯著差異,但蚵岩螺則無。第三部分為移地放養對葡萄牙牡蠣溶酶體膜不穩定度、肥滿度指數與一維電泳蛋白質體表現的影響,結果顯示溶酶體膜不穩定度於不同放養地點有顯著差異 (七股、台西1與台西2),七股最低;肥滿度指數整體有微幅上升之趨勢,且第7天與第14天三地間有顯著差異;一維電泳蛋白質體結果顯示第0天與第14天三地之葡萄牙牡蠣分為兩個類群 (Clade),PC1解釋了72.5 %的變異度,是主要分群的依據。整體而言,綠殼菜蛤、葡萄牙牡蠣、蚵岩螺三物種皆因時空變化而有生物指標表現變化,尤其以地點不同最為明顯。三種生物指標以溶酶體膜不穩定度所呈現時空變化的影響最可能與環境汙染有關,肥滿度指數之時空變化可能與環境食物量與生殖週期較相關,而一維電泳蛋白質體表現量無法反映環境與時間變動的影響。
Abstract
The present study was to evaluate the feasibility of three biomarkers, i.e. lysosomal membrane destabilization (LMD), condition index (CI) and 1D protein expression in environmental monitoring in southwest coast of Taiwan. Experiments were in three parts, firstly, the comparison of LMD in Perna viridis collected from different dates and sites, including Kaohsiung Harbor, Dapeng Bay and Sizihwan. Secondary, the differences of LMD and CI values in Crassostrea angulata and Thais clavigera among sites were investigated. Significant differences in LMD and CI were found in C. angulata. However, in T. clavigera, the difference was only in LMD. Thirdly, the LMD, CI value and 1D protein expression in transplanted C. angulata were determined. Spatial variation in LMD was observed and the lowest one was Qigu group. Slightly increases in CI value in all the experimental groups were detected. After oysters transplanted for 7 and 14 days, significant differences in LMD among sites were found. The 1D protein expression profiles were separated all the oyster samples into two clades and the PC1 explained 72.5% of the variation. Overall, the biomarkers of LMD, CI and 1D protein expression profiles were affected by temporal and spatial variations in mollusks of Perna viridis, C. angulata and T. clavigera. The temporal and spatial variations of LMD are mostly correlated to environmental pollution. The variations of CI may be mainly influenced by food abundance and reproduction. And, the 1D protein expression profiles couldn’t adequately reflect environmental variations.
目次 Table of Contents
圖次 頁碼
謝辭 I
中文摘要 II
英文摘要 III
表目錄 VII
圖目錄 VIII
一、前言 1
1.1 環境 1
1.2 軟體動物 1
1.3 生物指標 3
1.4 研究動機 5
二、材料與方法 6
2.1 比較不同時間地點綠殼菜蛤溶酶體膜不穩定度之差異 6
2.2 比較不同地點葡萄牙牡蠣與蚵岩螺溶酶體膜不穩定度與CI值之差異 7
2.2.1 溶酶體膜不穩定度測量 7
2.2.2 CI值計算與分析 7
2.3 移地放養對葡萄牙牡蠣溶酶體膜不穩定度、CI值與一維電泳蛋白質體表現的影響 8
2.3.1 溶酶體膜不穩定度測量 8
2.3.2 CI值計算與分析 9
2.3.3 一維電泳蛋白質體表現 9
三、結果 11
3.1 比較不同時間地點綠殼菜蛤溶酶體膜不穩定度之差異 11
3.2 比較不同地點葡萄牙牡蠣與蚵岩螺溶酶體膜不穩定度與CI值之差異 11
3.2.1 不同地點葡萄牙牡蠣與蚵岩螺溶酶體膜不穩定度之差異 12
3.2.2 不同地點葡萄牙牡蠣與蚵岩螺CI值之差異 12
3.3 移地放養對葡萄牙牡蠣溶酶體膜不穩定度、CI值與一維電泳蛋白質體表現的影響 13
3.3.1 溶酶體膜不穩定度 13
3.3.2 CI值計算與分析 13
3.3.3 一維電泳蛋白質體表現 14
3.4 總結 14
四、討論 15
4.1 溶酶體膜不穩定度生物指標 15
4.2 CI值生物指標 17
4.3 一維電泳蛋白質體表現生物指標 18
4.4 結論 20
五、參考文獻 21
表 25
圖 30
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