本研究以超音波噴頭結合乳化方式 (Emulsion process)，經由高分子材料聚己內酯 (Polycaprolactone, PCL) 製備出高粒徑均勻度之功能性微載體。PCL具有良好的生物降解性 (Biodegradability) 、生物相容性 (Biocompatibility) 、低熔點及高結晶性等特性，非常適合做於藥物之載體，研究過程藉由功率、幫浦進給速率、磁石轉速、聚乙烯醇 (Polyvinylalcohol, PVA) 濃度、PVA溶液溫度以及液滴滴落距離六種不同的參數，結合均勻設計實驗法 (Uniform design experimentation) 探討在不同的實驗條件下，微載體粒徑分佈的範圍及均一性，主要透過進給幫浦將PCL溶液以等速方式輸出至超音波噴頭，利用超音波噴頭表面所產生的駐波，將PCL溶液以液滴的方式滴落至下方PVA溶液，並在PVA溶液中加入磁石進行攪拌，使得PCL溶液析出溶液，產生PCL微載體，透過UV分光光譜儀建立檢量線，進而量測載藥率、包覆率、降解率以及釋放率，研究結果表示在PCL溶液濃度11.5 wt%、功率12.1 W、幫浦進給速率1.42 ml/min、磁石轉速690 rpm、PVA溶液濃度 13.3 wt%、PVA溶液溫度49 oC以及液滴滴落距離36.16 mm的參數下，可以製備出66.66 %均勻度，粒徑範圍約在30~50 μm之球狀載體，此載體之載藥率為7.265 %、包覆率為23.685%、釋放率在48小時達到2.1 %、降解率呈非線性在第14天時可達到80 %。

In this study, functional Polycaprolactone (PCL) microcarriers with high uniformity of particle size are prepared by ultrasonic nozzles. PCL has some good property for carrier of drug such as biodegradability, biocompatibility, low melting point and high crystallinity. Power, pump feeding rate, magnet rotation speed, the concentration of Polyvinylalcohol (PVA) solution, temperature, and droplet distance are the parameter used in the study. The range of particle size under different experimental condition is investigated by the uniform design experiment. PCL solution is delivered to the nozzle with constant speed by the infusion pump. Then PCL solution is sprayed into PVA solution by using the standing wave on the surface of ultrasonic nozzle. PVA solution stirred with magnet to separate out the microcarrier. The drug loading rate, coating rate, degradation rate, and release rate with the UV spectrophotometer was obtained. The results show that the size of microcarrier particle between 30 to 50 μm are produced by the parameters of PCL solution when the concentration is 11.5 wt%, power is 12.1 W, pump feeding rate is 1.42 ml/min, magnet speed is 690 rpm, PVA solution concentration is 13.3 wt%, temperature is 49 oC, and collecting distance is 24 mm. The uniformity of the microcarrier is 66.66% was obtained by above condition. And the drug loading rate is 7.265%, the coating rate is 23.685%, the degradation rate is reached to 80% at 14 days, and the release rate is reached to 2.1% at 48 hours.

第一章 緒論 5
1.1前言 5
1.2研究背景與動機 5
1.3研究目的 6
1.4本文架構 6
第二章 文獻回顧 7
2.1高分子材料-聚己內酯 (PCL) 7
2.2超音波噴頭 8
2.3高分子微球製程 10
2.4藥物載體應用 12
2.5生物降解 14
第三章 研究方法與步驟 19
3.1實驗流程架構 19
3.2實驗藥品與設備 19
3.3實驗製程 20
3.3.1乳化法溶液配置 20
3.3.2超音波乳化法製備 21
3.4均勻設計實驗法 22
3.4.1均勻設計表 22
3.4.2均勻實驗程序 25
3.4.3均勻設計實驗結果分析 25
3.5微載體特性量測 26
3.5.1檢量線建立 27
3.5.2載藥率量測 28
3.5.3包覆率量測 29
3.5.4釋放率量測 30
3.5.5降解率量測 30
3.6實驗分析儀器介紹 31
3.6.1流變儀 31
3.6.2掃描式電子顯微鏡 32
3.6.3表面張力量測儀 33
3.6.4 UV分光光譜儀 33
第四章 實驗結果與討論 35
4.1均勻設計實驗法分析 35
4.1.1均勻設計實驗法分析之PCL微球粒徑百分比 35
4.1.2均勻實驗設計法-線性回歸 45
4.1.3超音波乳化法PCL微載體製程參數最佳化分析 45
4.1.4最佳化參數之超音波乳化法PCL微球製程 46
4.2微載體特性量測結果分析 47
4.2.1載藥率及包覆率比較 47
4.2.2釋放率比較 50
4.2.3降解率比較 58
第五章 結論與未來展望 62
5.1結論 62
5.2未來展望 63
參考文獻 64

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